Sperm test / What is the Kruger Method?
There are several different criteria in sperm evaluation. “Kruger criteria” is a microscopic evaluation method that specifically considers deformities in sperm.
The selection of sperm cells with normal and close to normal morphology is extremely important in the treatment of infertility. For example, since the acrosome is an important site that contains the enzymes necessary for the sperm to enter the egg, acrosome abnormalities cause problems in the attachment of the sperm to the egg.
Nuclear abnormalities mean DNA content disorder. This prevents the development of a healthy embryo. Cytoplasmic droplet means that the sperm is not mature. Globozospermia means the absence of acrosomes, which means that sperm with such anomaly do not have the ability to fertilize.
The middle part is an important part where the mitochondria are. Abnormalities here cause a lack of energy support. This situation directly affects the mobility. When the tail of the sperm does not come out of the middle of the sperm head, that is, when the axis is broken, the ability of the sperm to move forward decreases.
Since the tail is the part that provides the motility and uprightness of the sperm, abnormalities in the tail have a restrictive effect on sperm movement. Kruger criteria are taken into consideration in semen morphological evaluation. Head, acrosome, nucleus, neck, tail and midpiece abnormalities are evaluated with these criteria.
Sperm test / According to Kruger
Fertilization success of sperm samples with 14% or more normal morphology is 88.3%
Fertilization success of sperm samples with normal morphology between 4-14% was 49.4%,
In cases where it is less than 4%, the fertilization success is 7.6%.
These data clearly demonstrate the correlation between morphology and fertilization. Knowing the semen parameters will shed light on which cases to inseminate, which cases IVF and which cases microinjection.
Anomalies in Morphology Determination by Sperm Test Kruger Method:
- Shape Anomalies
- Tapered, Elonge, Piriform, Round
- Macrocephaly, Microcephaly
- Diadem defect
- Invagination into the nucleus (usually in the equatorial region)
- Nucleus vacuoles
- Nucleus Size Anomalies
- Cytoplasmic Droplet
- Incomplete spermatid separation
- Usually binucleated, diploid, triploid
- Duplication
Abnormalities that may occur in the sperm test, Acrosome section are listed below:
- Primary (Occurring during development and differentiation)
- Irregular acrosomal distribution
- Acrosomal cyst
- Nipple, Switchboard
- Aberrant acrosomal structure
- Secondary (External factors, old age)
- Acrosomal content reduction (light staining)
- Total loss of acrosomal content
- Acrosomal membrane anomalies
- Acrosome separation
- Complete, Incomplete
Anomalies that may occur in the mid-piece are listed below:
- Cytoplasmic droplet
- Bend Mid-piece
- Kinked Mid-piece
- Amorphous Mid-piece
- Non-axial Mid-piece
- Mitochondrial aplasia
- Complete, Segmental
- Pseudodroplet
- Detached head
Anomalies that may occur in the queue are listed below:
Other anomalies other than the anomalies described above are listed below:
There is a special sampling room in our Şişli and Kadıköy branches for
Spermiogram test. It is possible to watch DVD while giving samples. You can also bring your own DVD.
While performing the spermiogram test, sperm shape (morphology) characteristics are examined after a special staining, and the fertility capacity of the sperm sample is determined. Since many factors such as smoking, alcohol, heat, drugs and infections affect sperm production, the analysis of abnormal samples should be repeated two or three times with an interval of one month. If a fertility problem is detected in the sperm analysis, further examination of the man is carried out in terms of physical and hormonal aspects.
Sperm production cycle is repeated every 2-3 months. In other words, a sperm produced will be secreted into the semen after 2-3 months. Likewise, harmful factors that the person encounters or useful drugs used for treatment can also affect sperm production at the end of 3 months. This process should be kept in mind when evaluating the Spermiogram test results.